Applications of Recombinant DNA Technology

What do you mean by DNA fingerprinting? Explain it through RFLP.

What are GMOs? Describe the method of development of transgenic plants.

Differentiate between direct and indirect method of gene transfer. Name one indirect method suitable for gene transfer in dicot plants.

What is molecular pharming? Give applications of transgenic animals in molecular pharming.

Give the procedure of development of recombinant subunit vaccines.

Write a short note on DNA vaccines.

Describe the advantages of monoclonal antibodies developed by rDNA technology over that developed by Hybridoma technology.

Briefly describe the development of Humulin through rDNA technology.

Write a short note on humatrope and Protropin.

Briefly describe the applications of rDNA technology in crop improvement.

List the ethical issues related to the use of transgenic animals?

What is the role of vaccinia virus in the development of recombinant vaccine?

Write a short note on recombinant therapeutic agents.

Write a short note on humanised antibodies.

Assertion: In hybridoma technology, B cells are fused with myeloma cells. Reason: Myeloma cells are immortal.

Assertion: In Humulin, polypeptide A and polypeptide B are linked with disulfide bridges. Reason: C peptide is removed from proinsulin to form biologically active insulin.

DNA fingerprinting depends on identifying specific:

Short stretch of DNA used to identify complementary sequences in a sample is called:

Variable number tandem repeat (VNTR) are:

Cry genes or Bt genes are obtained from: