Genome Technology and Engineering
Class 12 · Bio Technology · 21 Questions
What is a genome? How is the genome of prokaryotes different from eukaryotes?
What are the types of genome mapping? Explain each with a comparative approach.
Suppose a DNA is extracted, purified and digested with enzyme BamH1. What type of mapping can be achieved? Mention the application of this type of mapping.
What is STS and its role in genome mapping?
Give a brief insight about the development of DNA sequencing technology and genomic workflow.
Discuss how next generation DNA sequencing technology has overcome the drawbacks of the first-generation DNA sequencing technology. Elaborate the methodology.
What is a unit of physical mapping? Discuss in detail.
Discuss the methodology and applications of third generation sequencing technology.
What are the different variations of Next Generation Sequencing? Explain in brief.
What are the applications of advanced sequencing technologies? Elaborate.
Discuss the applications of metagenomics.
What are the goals of genome engineering? Explain each in detail.
One of the applications of genome engineering is to achieve high level recombinant protein production. How can we achieve this? Explain in detail.
What is a genome editing? Why is there a need to edit a genome? Explain the methodology of editing using CRISPR-Cas9.
What are the structural, functional and comparative genomics?
What are the applications of protein engineering? Discuss in brief.
If you are given a recombinant protein with 6-His-tags, how are you going to use it? Explain the methodology and application in detail.
How do you apply protein engineering to track cellular localisation of proteins?
How to generate a recombinant immunotoxin? Why do you think there is a need for generation of an immunotoxin? Mention its mechanism of action.
Assertion: RFLP is variation in length of DNA fragments when cleaved by same restriction endonuclease. Reason: Genome of every individual has different position of restriction sites.