What are the applications of advanced sequencing technologies? Elaborate.
With the development of high-throughput DNA sequencing, DNA sequencing technology has been adapted to do much more than just reveal the genome sequence of an organism. One can use this technology to study the biology of an organism at genome level.
ChipSeq sequencing projects are used to map genome-wide DNA binding sites of a transcription regulator. Since transcription regulators play an important role in controlling the gene expression of one or more genes, ChipSeq projects help identify genome-wide DNA binding sites as well as target genes of a transcription regulator.
RNASeq projects are carried out to study the global gene expression profile (transcriptome) of an organism, a tissue, or a sample. In this method, total RNA is extracted from the target sample and cDNA is synthesised, which is then sequenced using next generation sequencing technologies.
Next generation sequencing includes techniques like whole genome sequencing (WGS), Targeted resequencing, and clinical exome sequencing. Additionally, Nanopore sequencing technology is simple, rapid, cost efficient, displays results in real-time, and is useful for genotyping and high mobility testing.
Explanation
The textbook context clearly outlines the applications of advanced sequencing technologies. The answer covers: (1) The broader application of studying biology at genome level, (2) ChipSeq for mapping transcription regulator binding sites, (3) RNASeq for transcriptome analysis, (4) Other NGS techniques like WGS and clinical exome sequencing, and (5) Nanopore sequencing applications. All information is drawn directly from the provided context without external knowledge.