Microbial Culture

Describe the nutritional requirements of microorganisms.

Write an account of the growth curve analysis of a microorganism.

Discuss any two methods to isolate a pure culture.

Give a detailed account on the various methods of sterilisation.

Who was the first to develop the process of colony purification on solid media?

HTST and UHT methods belong to:

Germ theory of disease was suggested by:

Calculate the specific growth rate and generation time of a bacterial population in which the number of bacteria increases from $10^{4}$ cells/mL to $10^{7}$ cells/mL, during 4 hours of exponential growth.

Assertion: A selection medium containing Ampicillin is sterilised by autoclaving. Both $amp^{S}$ and $amp^{R}$ microbes show growth on the medium. Reason: To inhibit the growth of $amp^{S}$ microbe ampicillin should not have been autoclaved but sterilised by using micro-filters before adding to the medium.

Assertion: Microorganisms are able to grow and multiply over a wide range of temperatures. Reason: Extreme thermophiles can tolerate temperatures of above 100°C.