Question 7 of 18intermediate💡 UnderstandShort Answer3 marks

Discuss the various types of organ culture.

Correct Answer

The various types of organ culture are whole embryo culture, histotypic culture, and organotypic culture.

Histotypic culture involves the proliferation of a characterised cell line at a high density. This process occurs in the presence of suitable soluble factors and an extracellular matrix. For example, vascular endothelial cells can form capillary tubules when grown in a collagen matrix, or cells may infiltrate cellulose sponges to form glandular structures.

Organotypic culture and whole embryo culture are other distinct types used to recreate the three-dimensional structure of organs for studying tissue behaviour and developmental biology.

Exercise: EXERCISES | Q: 8 | (Page 20)
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Explanation

The answer is derived directly from the textbook context which explicitly lists the three types of organ culture: whole embryo culture, histotypic culture, and organotypic culture. The explanation for histotypic culture is detailed in the text, mentioning high-density proliferation and examples like collagen matrices. The other types are listed to ensure the answer is comprehensive as per the source material.