Question 15 of 16intermediate💡 UnderstandMCQ1 mark

Which of the following components is not essential for Murashige and Skoog media?

Exercise: EXERCISES - Multiple Choice Questions | Q: 15 | (Page 17)
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Explanation

Option C (Antibiotics) is correct because when we examine the composition table for Murashige and Skoog (MS) media provided in the context, it lists macronutrients, micronutrients (both inorganic nutrients), sucrose (carbon source), and organic supplements like vitamins (thiamine, pyridoxine, nicotinic acid), myoinositol, and glycine. However, antibiotics are NOT listed anywhere in the MS media composition table. While the text mentions that culture media 'broadly contain' six components including antibiotics, the actual MS media composition table does not include antibiotics as an essential component. The other three options (Inorganic nutrients, Carbon source, and Organic Nutrients) are all clearly present in the MS media composition.

Solution Steps

  1. Step 1: Examine the MS media composition table to identify all listed components.

  2. Step 2: Verify presence of Inorganic nutrients (macronutrients and micronutrients are listed).

  3. Step 3: Verify presence of Carbon source (sucrose is listed at 30 mg l⁻¹).

  4. Step 4: Verify presence of Organic Nutrients (vitamins, myoinositol, glycine are listed).

  5. Step 5: Check for Antibiotics in the composition table - NOT found.

  6. Step 6: Conclude that Antibiotics is the component not essential for MS media.

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