What are the major characteristics of a vector?
The major characteristics of a vector are:
First, a vector should be small in size for easy incorporation into the host cell, yet be able to integrate a large size of the insert. This ensures efficient transfer and accommodation of the foreign DNA.
Second, the vector must have an origin of replication (ori) so that it is capable of autonomous replication inside the host organism. This allows the vector to multiply along with the inserted gene.
Third, the vector needs to have unique restriction sites. If it possesses too many restriction sites, it would be fragmented into several pieces during the cloning process.
Finally, the vector requires at least one selectable marker to screen out transformants. Examples include antibiotic resistance genes such as tetracycline () and ampicillin ().
Explanation
The answer covers all four major characteristics of a cloning vector as mentioned in the NCERT textbook: small size with large insert capacity, origin of replication, unique restriction sites, and selectable markers. These features are essential for successful gene cloning and are explicitly listed in both the main text and the summary section of the provided context.