Gene Cloning

Class 12 · Bio Technology · 17 Questions

1intermediate💡 Understand5 marksLong Answer

Describe the methods used for isolation of DNA.

2beginner💡 Understand2 marksShort Answer

What is the role of biological detergent in the process of isolation of nucleic acid?

3intermediate🔍 Analyze3 marksShort Answer

How does DNA isolation from plant tissue differ from that of bacterial cell?

4intermediate💡 Understand3 marksShort Answer

How many types of restriction enzymes (REs) are there? Can all REs be used in rDNA technology? Give justification.

5beginner🧠 Remember2 marksShort Answer

What are the challenges faced during the process of nucleic acid extraction?

7beginner💡 Understand2 marksShort Answer

Describe the role of chelating agent in the process of DNA extraction.

8intermediate💡 Understand5 marksLong Answer

Briefly describe the modes of DNA transfer into the host.

9intermediate💡 Understand1 markMCQ

Identify the correct statement for blue–white selection method.

10beginner🧠 Remember1 markMCQ

Identify the correctly matched pair from the following options.

12advanced💡 Understand5 marksLong Answer

How are recombinants screened? Describe the methods in detail.

13intermediate🔍 Analyze5 marksLong Answer

Differentiate between the Southern, Northern and Western blotting.

14intermediate💡 Understand5 marksLong Answer

What is PCR? Describe in detail.

15intermediate🔍 Analyze5 marksLong Answer

Write a comparative account of the genomic and cDNA libraries.

16beginner🧠 Remember1 markMCQ

Diploid human genome contains:

17intermediate💡 Understand1 markMCQ

Select the incorrectly matched pair from the following.

18intermediate🔍 Analyze1 markMCQ

Assertion: PCR can be used to amplify very small amount of DNA using DNA modifying enzymes. Reason: PCR uses Taq Polymerase.

19intermediate🔍 Analyze1 markMCQ

Assertion: Foreign gene can be introduced into host bacterium by transformation techniques like electroporation. Reason: Bacteria have cell wall/membrane.