Question 5 of 6beginner🧠 RememberMCQ1 mark

A culture in a closed vessel to which no additional medium is added is called _____ culture.

Exercise: EXERCISES | Q: 9 | (Page 22)
For More Understanding

Explanation

Option (B) Batch is correct. A batch culture is characterized by a closed vessel system where no additional medium is added during the culture process. The context clearly distinguishes between different culture modes: in continuous mode, fresh nutrient media is added and used media is removed continuously; in fed-batch mode, substrate components are fed intermittently or continuously to the growing culture. Since the question specifically describes a closed vessel with no additional medium added, this matches the definition of batch culture. The graphical representation in the context also shows batch mode as the baseline culture system where the culture grows without external medium addition until nutrients are depleted.

Solution Steps

  1. Step 1: Identify the key characteristics in the question - closed vessel and no additional medium added.

  2. Step 2: Compare with culture modes described in context - Continuous mode involves adding fresh media and removing used media; Fed-batch involves feeding substrate intermittently.

  3. Step 3: Conclude that Batch culture is the only mode where no additional medium is added in a closed vessel system.

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