Question 4 of 21intermediate🧠 RememberShort Answer3 marks

Describe any two chemically synthesised media.

Correct Answer

Two chemically synthesised media are described below:

Chemically defined media (Basal media) include Phosphate buffered saline (PBS), Dulbecco's phosphate buffered saline (DPBS), Hank's balanced salt solution (HBSS), and Earle's balanced salt solution (EBSS). These are composed of inorganic salts that maintain the physiological pH and osmotic pressure. The inorganic ions play a physiological role in maintaining the membrane potential.

Protein-free media use undefined components such as peptide fractions (protein hydrolysates) as supplements. These media do not contain defined proteins but rely on protein hydrolysates to provide necessary nutrients for cell culture.

Exercise: EXERCISES | Q: 4 | (Page 22)
For More Understanding

Explanation

The question asks for any two chemically synthesised media. From the textbook context, under 'Chemically defined media', several basal media are listed (PBS, DPBS, HBSS, EBSS) with their composition and function clearly described. Protein-free media is another category where undefined components like peptide fractions are used. Both are valid examples of chemically synthesised media mentioned in the NCERT textbook.

Solution Steps

  1. Step 1: Identify chemically synthesised media types from the context - Chemically defined media and Protein-free media

  2. Step 2: Describe Chemically defined media with examples (PBS, DPBS, HBSS, EBSS) and their composition/function

  3. Step 3: Describe Protein-free media with its key characteristic (use of peptide fractions/protein hydrolysates)

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