Yes, the impact of removing all organisms in a trophic level will be different for different trophic levels. The producers form the lowest trophic level and there are generally a greater number of individuals at lower trophic levels. Since producers make energy from sunlight available to the rest of the ecosystem, removing them would have a greater impact than removing organisms from higher trophic levels. There is also a loss of energy as we go from one trophic level to the next, which affects the number of organisms at each level.

No, the organisms of any trophic level cannot be removed without causing damage to the ecosystem. The various components of an ecosystem are interdependent. Each organism is generally eaten by two or more other kinds of organisms, which in turn are eaten by several other organisms, forming a food web. This complex interrelationship means that removing any trophic level would disrupt the ecosystem and affect other organisms dependent on it.