Molecular Detective: Blotting & Selection Lab
Gene Cloning
Step into the CCMB Hyderabad Molecular Diagnostics Unit to master blue-white selection and blotting techniques. Learn how scientists identify recombinant colonies and detect specific DNA, RNA, and proteins through interactive visualizations.
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📖 Your Story
You are a research intern at the Centre for Cellular and Molecular Biology (CCMB), Hyderabad, one of India's premier research institutes. Your mentor Dr. Padma has assigned you to the diagnostics wing where you'll learn the critical techniques of selecting recombinant bacteria and identifying biomolecules using blotting methods.
What You'll Learn
- Understand the principle of blue-white selection using lacZ gene and X-gal substrate
- Differentiate between Southern, Northern, and Western blotting techniques
- Identify appropriate detection methods (probes, antibodies) for each blotting type
- Apply knowledge to select the correct technique for specific molecular detection scenarios
How to Play
3 Missions Await
Colony Screening Challenge
4 problems · 40 XP
Blotting Technique Explorer
4 problems · 60 XP
Diagnostic Applications Master
3 problems · 60 XP